試劑耗材

Viromer來自德國,為創(chuàng)新型轉(zhuǎn)染試劑而生,且只做轉(zhuǎn)染試劑,只賣轉(zhuǎn)染試劑,因為專業(yè),所以值得信賴!
如果您需要轉(zhuǎn)染的細胞非常難轉(zhuǎn),多次轉(zhuǎn)染不成功的話,建議嘗試Viromers®轉(zhuǎn)染試劑,它是基于病毒融合機制的聚合物,該機制以流感病毒血凝素的融合肽為藍本;當暴露于低pH時,融合肽失電荷,變?yōu)槭杷,進入核膜。推薦用于原代細胞,懸浮細胞,分化中細胞或干細胞等難轉(zhuǎn)細胞。
Viromers ® RED 和Viromers ® YELLOW 均適用于質(zhì)粒和mRNA的轉(zhuǎn)染,兩者分別適用于不同細胞系。Viromer® RED適用范圍更廣。初次轉(zhuǎn)染時,建議轉(zhuǎn)染試劑(uL):DNA(ug)=1:2.5-3.5。
- 避免包涵體吞噬:最大化轉(zhuǎn)染效率并降低背景;
- 零電荷:無需去除血清和抗生素,對細胞溫和;
- 無脂質(zhì):適用于脂肪代謝活躍的細胞,如:脂肪細胞或肝細胞。
- 穩(wěn)定:實驗結(jié)果具有重復(fù)性;
- 反向轉(zhuǎn)染:適用于高通量篩選。
產(chǎn)品特點:
高轉(zhuǎn)染效率,高細胞存活率。

Viromers® RED /YELLOW 與jetPRIME, FuGENE, Lipofectamine 及 Mirus X2 的轉(zhuǎn)染效率比較
使用Viromer® RED /YELLOW及其它轉(zhuǎn)染試劑,轉(zhuǎn)染pCMV-Luc (50ng DNA/96-well)到標準細胞系中,檢測熒光素酶表達及細胞存活率,發(fā)現(xiàn)Viromers® RED /YELLOW轉(zhuǎn)染Hela,CHO,HEX獲得更高的轉(zhuǎn)染效率。
應(yīng)用實例1
SH-SY5Y neuroblastoma

使用Viromer®RED, FuGene ®HD or TransIT-X2™ 轉(zhuǎn)染eGFP 質(zhì)粒 (100ng DNA/96-well)到SH-Sy 5yneuroblastoma,24h后的FACS及鏡檢結(jié)果
應(yīng)用實例2
RAW 264.7 mouse macrophages

使用Viromer®RED, FuGene ®HD or TransIT-X2™ 轉(zhuǎn)染eGFP 質(zhì)粒 (100ng DNA/96-well)到RAW264.7 mouse macrophages,24h后的FACS及鏡檢結(jié)果
訂購信息:
| 貨號 | 品名 | 規(guī)格 | 價格 | 備注 |
| VR-01LB-00 | Viromer® RED | 80 T in 24 well | 1000 | 已驗證細胞系 參照表3 |
| VR-01LB-01 | 0.18 ml(600T) | 7140 | ||
| VR-01LB-03 | 3 x 0.18 ml(1800T) | 18360 | ||
| VY-01LB-00 | Viromer® YELLOW | 80 T in 24 well | 1000 | |
| VY-01LB-01 | 0.18 ml(600T) | 7140 | ||
| VY-01LB-03 | 3 x 0.18 ml(1800T) | 18360 |
表3. Viromer® RED & YELLOW cell 驗證細胞系
| Category | Cell type | Viromer® |
| Cell lines | Standards: HeLa, Neuro2A, CHO, HepG2, HEK-293 … | RED & YELLOW |
| HT-29 colorectal carcinoma | RED | |
| A549 lung adenocarcinoma | RED | |
| H226 lung squamous cell carcinoma | RED | |
| BEAS-2B bronchial epithelial | RED | |
| HACAT keratinocytes | RED | |
| Huh-7 hepatocellular carcinoma | RED | |
| SH-SY5Y neuroblastoma | RED | |
| INS-1 insulin secreting beta cell derived line | YELLOW | |
| Suspension cells | THP-1 monocytes | RED |
| Primary & Stem cells | Primary mouse hepatocytes | RED |
| Primary keratinocytes | RED | |
| Primary human macrophages | RED | |
| Primary cardiomyocytes | YELLOW | |
| Primary fibroblasts | YELLOW | |
| Primary myoblasts | RED & YELLOW |





浙公網(wǎng)安備 33010402001362號